SpaK/SpaR Two-component System Characterized by a Structure-driven Domain-fusion Method and in Vitro Phosphorylation Studies
Author Summary Because proteins so frequently function in coordination with other proteins, identification and characterization of the interactions among proteins are essential for understanding how proteins work. Computational methods for identification of protein-protein interactions have been limited by the degree to which proteins are similar in sequence. However, methods that leverage structure information can overcome this limitation of sequence-based methods; the three-dimensional information provided by structure enables identification of related proteins even when their sequences are dissimilar. In this work we present a quantitative method for identification of protein interacting partners, and we demonstrate its use in modeling the structure of a hypothetical complex between two proteins that function in a bacterial signaling system. This quantitative approach comprises a tool for generation of hypotheses regarding protein function, which can then be tested using empirical methods, and provides a b
Jun 5, 2009 ... Virginia Rodriguez,. Affiliation Genome Technology Branch, National Human Genome Research Institute, National Institutes of Health, ...